Which method can be utilized for ASF diagnosis apart from virus isolation?

The appropriate method for diagnosing African Swine Fever (ASF) apart from virus isolation is the enzyme-linked immunosorbent assay (ELISA). This technique is particularly valuable due to its ability to detect ASF virus antigens or antibodies in blood samples. ELISA is favored because it is relatively quick, sensitive, and specific, allowing for effective screening of large populations, which is crucial in controlling the spread of ASF.

Other methods, while potentially useful in different contexts, do not provide the same level of reliability or applicability for ASF diagnosis. For example, HAD tests may not specifically target the ASF virus, and necropsy examinations may offer insights into general health status but are not definitive for ASF without additional testing. Similarly, assessing animal behavior can provide indications of illness but lacks the scientific rigor needed for concrete diagnostic purposes. Thus, ELISA stands out as the most suitable method when isolating the virus is not feasible.