Which method is used to test for Viral Newcastle Disease (VND)?

The method to test for Viral Newcastle Disease (VND) that is recognized as the most reliable is viral isolation via embryonate Specific Pathogen-Free (SPF) eggs. This approach involves inoculating the embryonated eggs with a sample suspected of containing the virus. If VND is present, the virus will replicate within the embryonated eggs, allowing for identification and diagnosis. This method is particularly effective because embryonated eggs provide a suitable environment for the virus to grow, making it possible to confirm the presence of VND definitively.

Other testing methods are not appropriate for diagnosing VND. Blood smear examinations, for example, are generally used to detect parasites or other blood-related issues rather than viral infections. Necropsy examinations may provide insights into the pathological effects of infections but do not specifically isolate or identify viruses like VND. Urine analysis is typically utilized for other types of diseases rather than diagnosing viral infections in poultry. Therefore, the isolation of the virus using embryonated SPF eggs stands out as the optimal method for confirming the presence of Viral Newcastle Disease.