Which of the following is NOT a typical method for diagnosing ASF?

Fluorescent microscopy is not a typical method for diagnosing African Swine Fever (ASF) because this technique primarily focuses on visualizing specific antigens or cellular structures within a sample using fluorescently labeled antibodies. While it can be useful in certain diagnostic contexts, it does not have the specificity or sensitivity required for the direct identification of ASF virus, which is crucial for accurate diagnosis of the disease.

In contrast, virus isolation techniques, serological tests, and molecular methods like PCR are all established methods for diagnosing ASF. Virus isolation allows for the assessment of live virus from samples, directly confirming the presence of the pathogen. Serological tests detect antibodies against the virus, indicating prior infection in the animal, while molecular methods such as PCR provide rapid and accurate identification of viral genetic material, making them highly effective for ASF diagnosis. Thus, the options that focus on viral detection and immune response are considered standard practice in the field for diagnosing ASF.